Become A Master Hplc – Analysis Specialist Training

What you'll learn
Comprehensive HPLC Knowledge: Participants will gain in-depth knowledge of HPLC technology.
Expert Training: Our expert trainers will guide participants in equipment usage, solution and analyte preparation, and analysis with different detectors.
Methodology and Process Understanding: A solid understanding of the methodologies and processes used in HPLC analysis.
Software Proficiency: Participants will develop proficiency in using the software associated with HPLC devices.
Problem-Solving Skills: Training will equip participants with the tools to address various challenges that may arise during analysis.
Independent Analysis: By the program's conclusion, participants will have the knowledge and confidence to conduct HPLC analysis independently.
Nuanced Understanding: Participants will grasp the intricacies of HPLC analysis, enabling them to analyze with confidence.
Requirements
Basic Knowledge of Chemistry, About Analysis, and Math
Description
BEST ONLINE HPLC TRAINING IN THE WORLD!High Performance Liquid Chromatography (HPLC) is a widely used analytical technique in the field of chemistry and biology. It is used to separate, identify and quantify components in a mixture. With the increasing demand for high-quality analysis, it is important to have the necessary skills and knowledge to operate HPLC equipment and interpret results accurately. This is where our HPLC Training comes in.Our comprehensive training program is designed to provide you with a complete understanding of HPLC and its applications. Whether you are a beginner or an experienced analyst, our courses offer a comprehensive understanding of the principles and practices of HPLC. You will learn the logic of HPLC, such as how the device works, what the different parts of the equipment are and how to operate it safely.One of the key features of our HPLC Training is that it is taught in a laboratory setting. This means you will see hands-on work with the equipment and learn how to apply a variety of analytical techniques. Also, our program includes 21 different analysis applications, so you can familiarize yourself with the various applications of HPLC and learn how to apply them in different scenarios.Throughout the training, you will be taught by expert trainers who have years of experience in HPLC analysis. They will guide you through every step of the process, from the preparation of solutions and analytes to the analysis itself. You will learn how to analyze with different detectors, understand the different types of problems that may arise and how to solve them.Our HPLC Training program also covers the software part of the device, so you will learn how to navigate and use the software to analyze data effectively. This includes mastering the calibration process, calibration curve creation, and the reporting and printing process.By the end of the training, you will have a thorough understanding of HPLC and be able to analyze independently with HPLC devices. You will also have the necessary skills to manage a laboratory and be a lab manager. Whether you are looking to advance your career in the field of chemistry or biology, or simply want to gain a deeper understanding of HPLC, our training program is the perfect solution. Enroll now and take the first step towards becoming an HPLC expert.
Overview
Section 1: Mobile Phase And Washing Solutions
Lecture 1 What is the Mobile Phase?
Lecture 2 Wash Solvents and Lines
Lecture 3 Recommendations For Different Washing Systems
Lecture 4 How to Connect Mobile Phase to the System
Lecture 5 Reverse Phase and Normal Phase
Section 2: HPLC Pump Module
Lecture 6 Operation System of Pump Module
Lecture 7 How to Operate the Pump System?
Lecture 8 How Should We Remove Air Bubbles Formed in the System?
Lecture 9 The Liquid Sensor
Lecture 10 The Pump Module in Different Brands of Devices
Section 3: Auto Sampler Module
Lecture 11 Auto Sampler Module
Lecture 12 Sample Vials
Lecture 13 How Does Work the Injection System
Section 4: Column Oven Module
Lecture 14 Column Oven
Lecture 15 The Column Advice For Wash Process
Lecture 16 HPLC Columns
Lecture 17 How Assembled The Column?
Lecture 18 What is the Guard Column?
Section 5: Preliminary Information About HPLC Detectors and Software System
Lecture 19 Detectors Used In HPLC Devices
Lecture 20 Let's Review The Software Program
Section 6: Things to Do Before Analysis
Lecture 21 Recommendations – Before Starting the Analysis
Lecture 22 How Much Should the Mobile Phase Amount Be?
Lecture 23 Preparation of the Mobile Phase
Lecture 24 Recommendations
Lecture 25 Things to Do Before Connecting the Mobile Phase and Washing Solutions
Lecture 26 Characteristics of Columns
Lecture 27 Pressure Problems Related to Columns
Lecture 28 Operation and Washing Processes of The Device – Auto Purge 1
Lecture 29 Cleaning the Pump Module
Lecture 30 Starting Flow in the Pump Module
Lecture 31 Connecting the Mobile Phase and Washing Solutions to the Device
Section 7: Preparation of Samples and Analytes
Lecture 32 Working Logic with Solutions
Lecture 33 Preparation of Reference Solutions
Lecture 34 Preparation of Stock Solution
Lecture 35 Preparation of Sample Solution
Lecture 36 Preparation of Stock Standard Solutions
Lecture 37 Preparation of Vials
Lecture 38 Placing Vials on the Tray
Section 8: Method Creation and Method Definition In The Software
Lecture 39 New method
Lecture 40 Creating the Batch Table
Section 9: How to Analyze with HPLC Device?
Lecture 41 Conditioning the Device and Column
Lecture 42 Starting to Analysis
Lecture 43 Setting the Shutdown Washing Mode
Section 10: Determination of Retention Times
Lecture 44 Determination of Retention Times of Reference Standards (Automatic Integration)
Lecture 45 Determination of Retention Times of Reference Standards (Manual Integration)
Lecture 46 Determination of Vitamin C Standard
Lecture 47 Determination of Vitamins B1, B3, B6
Section 11: Calculation of Actual Concentrations
Lecture 48 Calculation of Concentrations
Section 12: Processing the Analysis
Lecture 49 Introduction to Processing Analysis Results
Lecture 50 Getting Started with the Process
Lecture 51 Processing the Batch Table
Lecture 52 Calling the Batch Table
Lecture 53 Adding Parameters to the Result
Section 13: Displaying the Calibration Curves
Lecture 54 Displaying the Curves
Section 14: Calculation Process
Lecture 55 Calculation
Section 15: Reporting, Calibration Curve And Print Process
Lecture 56 Control of Concentrations Before Reporting
Lecture 57 Report Preparation
Lecture 58 Calibration Curve Deviate
Lecture 59 Printing in a Different Format
Lecture 60 Setting Peaks in a Report
Section 16: Analysis in a Gradient System
Lecture 61 Analysis in a Gradient System
Section 17: Washing Processes / Auto Purge
Lecture 62 Operation and Washing Processes of The Device Auto Purge Lesson 1
Lecture 63 Washing Processes Auto Purge – Lesson 2
Section 18: Mixing of Mobile Phases in Different Ways
Lecture 64 Mixing in the Pump Line
Lecture 65 Comparing Mixtures
Section 19: Modifying the Method And Batch While the Analysis Is In Progress
Lecture 66 Modifying the Method While the Analysis Is In Progress
Lecture 67 Making Changes to the Batch Table While Analysis Is In Progress
Section 20: Diode Array Detector || Analysis And Interpreting Results
Lecture 68 Preparing Device and Lines Before Analysis in Diode Array Detector
Lecture 69 Analysis in a Diode Array Detector
Lecture 70 Interpreting Results in a Diode Array Detector
Section 21: Analysis With RID Detector
Lecture 71 Analysis in RID Detector
Section 22: Different Columns With The Same Feature
Lecture 72 Analysis in Fluorescent Detector
Section 23: Different Columns With The Same Feature
Lecture 73 Analysis with 25 cm Long Column
Lecture 74 Long Column vs. Short Column
Section 24: Problems That May Be Encountered And Solution Methods
Lecture 75 Tailing Problem
Lecture 76 Resolution – Dividing Peak Retention Times
Lecture 77 If Parameters Change
Lecture 78 If Flow Rate Changes
Lecture 79 If the Wavelength Changes
Lecture 80 If the UV Detector Lamp Stays Off
Lecture 81 If Column Oven Temperature Changes
Lecture 82 Lamp Life of UV Detector
Lecture 83 If There Is a Difference in Mobile Phase Ratio
Laboratory Professionals,Students,Method Develeopers,Scientists,Analysts and laboratory personnel aiming to acquire troubleshooting skills to address potential issues.,Professionals working in laboratory environments who want to enhance their management skills.,Participants interested in learning the pre-analytical steps and precautions required in HPLC.,Individuals who wish to have a thorough understanding of the methodologies and processes involved in HPLC analysis.,Anyone who wants to become master at using HPLC software for data analysis.,Analysts and scientists aiming to gain proficiency in analyzing samples with different types of detectors.,Those seeking knowledge about the preparation of solutions and analytes for HPLC analysis.,Individuals who want to learn about HPLC equipment and its proper usage.,Professionals looking to gain a comprehensive understanding of HPLC analysis techniques.

Homepage

https://www.udemy.com/course/become-a-master-hplc-analysis-specialist-training/